Journal: eLife

Article Title: Critical roles for ‘housekeeping’ nucleases in type III CRISPR-Cas immunity

doi: 10.7554/eLife.81897

Figure Lengend Snippet:

Article Snippet: Escherichia coli DH5α was propagated in Luria-Bertani (LB) broth (VWR, PA), and E. coli BL21 (DE3) was propagated in Terrific broth (TB) medium (VWR) for protein purification.

Techniques: CRISPR, Isolation, Recombinant, Over Expression, Purification, Sequencing, Construct, Plasmid Preparation, Protein Purification, Software

Journal: Protein and Peptide Letters

Article Title: Boosting Auto-Induction of Recombinant Proteins in Escherichia coli with Glucose and Lactose Additives

doi: 10.2174/0929866528666210805120715

Figure Lengend Snippet: Effect of additives on recombinant GFPuv production using auto-induction. ( A ) Final OD 600 and final pH of the culture media. OD 600 , grown without additives ( white bar ); OD 600 , grown with additives ( gray bar ); pH, grown without additives ( open square ); pH, grown with additives ( open circle ). ( B ) Relative fluorescence per cell density. ( C ) Relative total fluorescence. The fluorescence values obtained without ( white bar ) or with ( gray bar ) additives are shown in the panels.Transformed E. coli cells were grown with six different culture media without additives, the results for which are shown on the left of each medium condition. Independently, four cultures with additives and two control cultures without additives were carried out, the results for which are shown on the right of each condition. The two independent cultures of each condition were subjected to statistical analysis using a Student’s t-test: *, P <0.05; NS, not significant. One-way analysis of variance followed by Dunnett's test was performed for comparison of culture media. a, P <0.05 compared to ON medium ( left ); b, P <0.05 compared to ON medium ( right ). Error bar indicates the standard deviation value. Statistical significance was identified in pH results for all conditions except TB-2 medium (not shown for clarity). Abbreviations: LB, Luria-Bertani; LBM, LB Broth Miller; OD 600 , optical density at 600 nm; ON, Overnight Express™ Instant TB; TB, Terrific Broth.

Article Snippet: Two types of Terrific Broth (TB) media were prepared in this study: TB-1 medium contained 12 g/L Polypepton (Nihon Pharmaceutical), 24 g/L yeast extract D-3 (Nihon Pharmaceutical), 11.7 g/L glycerol, 9.4 g/L K 2 HPO 4 , and 2.2 g/L KH 2 PO 4 , while TB-2 medium contained 12 g/L tryptone (Nacalai Tesque), 24 g/L yeast extract D-3 (Nihon Pharmaceutical), 11.7 g/L glycerol, 9.4 g/L K 2 HPO 4 , and 2.2 g/L KH 2 PO 4 .

Techniques: Recombinant, Fluorescence, Transformation Assay, Control, Comparison, Standard Deviation

Journal: Protein and Peptide Letters

Article Title: Boosting Auto-Induction of Recombinant Proteins in Escherichia coli with Glucose and Lactose Additives

doi: 10.2174/0929866528666210805120715

Figure Lengend Snippet: Effect of additives on recombinant oANG production using auto-induction. ( A ) Final OD 600 and final pH of the culture medium. OD 600 , grown without the additives ( white bar ); OD 600 , grown with the additives ( gray bar ); pH, grown without the additives ( open square ); pH, grown with the additives ( open circle ). ( B ) oANG amount per cell density. ( C ) Total oANG amount. The amounts obtained without ( white bar ) or with ( gray bar ) additives are shown in the panels. The cultivation design, as well as, graphical representation of results are the same as in the case of GFPuv. The details of statistics analysis are same as those described in the legend for Figure . Statistical significance was identified in pH results for all conditions except TB-1 and TB-2 media (not shown). LB, Luria-Bertani; LBM, LB Broth Miller; oANG, ovine angiotensinogen; OD 600 , optical density at 600 nm; ON, Overnight Express™ Instant TB; TB, Terrific Broth.

Article Snippet: Two types of Terrific Broth (TB) media were prepared in this study: TB-1 medium contained 12 g/L Polypepton (Nihon Pharmaceutical), 24 g/L yeast extract D-3 (Nihon Pharmaceutical), 11.7 g/L glycerol, 9.4 g/L K 2 HPO 4 , and 2.2 g/L KH 2 PO 4 , while TB-2 medium contained 12 g/L tryptone (Nacalai Tesque), 24 g/L yeast extract D-3 (Nihon Pharmaceutical), 11.7 g/L glycerol, 9.4 g/L K 2 HPO 4 , and 2.2 g/L KH 2 PO 4 .

Techniques: Recombinant

Journal: Micromachines

Article Title: Cross-Flow Filtration of Escherichia coli at a Nanofluidic Gap for Fast Immobilization and Antibiotic Susceptibility Testing

doi: 10.3390/mi10100691

Figure Lengend Snippet: Fluorescent microscope images (20× magnification) showing E. coli immobilized at the nanogap at 37 °C with Terrific Broth (TB) medium flowing through the detection channel over 4 hours. ( a – c ) Three exemplary growth experiments designated A, B, and C. Measured cells are numbered within each experiment. ( d ) Negative control with addition of antibiotics (200 µg/mL kanamycin). Marked are measured cells.

Article Snippet: Bacterial dilutions in the range of 10 5 to 10 7 cells/mL were prepared in Terrific Broth (TB) growth medium (Lionex GmbH, Braunschweig, Germany) containing 100 μg/mL carbenicillin (beta-lactam-antibiotic) and 10 μg/mL gentamicin-sulfate, which was also used as culture medium.

Techniques: Microscopy, Negative Control